Peroxisomes do it, too

There is hardly any cellular organelle not involved in calcium handling. Mitochondria do it, the Endoplasmic reticulum does it, lysosomes do it, too, and now peroxisomes join the club. Yelena with help by MD student Uta and in a collaborations with Katrin Streckfuß-Bömeke and Ivan Bogeski completed a manuscript that we published in BioRxiv (Bioarchive). Yelena and Uta made FRET sensors for looking at peroxisomal calcium. They used them in HeLa cells and cardiomyocytes (from rat and human induced pluripotent stem cell-derived cardiomyocytes). They showed that peroxisomes in HeLa cells take up calcium upon depletion of intracellular calcium stores and upon calcium influx across the plasma membrane. Also peroxisomes in cardiomyocytes can take up calcium in a controlled manner. Peroxisomal and cytosolic calcium signals are tightly interconnected and peroxisomes may play an important role in shaping cellular calcium dynamics by serving as buffers or sources of intracellular calcium.

Measuring calcium influx into the peroxisome